Method to make paraffin‐embedded breast and lymph tissue mimic fresh tissue in DNA analysis

Absence of Human Papillomavirus in Benign and Malignant Breast Tissue

Background & Objective: Malignant breast tumors, which are one of the most important deadly cancers in women, like many other cancers, are proposed to be related to viruses etiologically. Proper management of breast carcinoma necessitates an identification of the etiological factors. Human Papillomavirus is considered to have an etiological role in breast carcinoma. We...

Tissue preparation for laser capture microdissection and RNA extraction from fresh frozen breast tissue.

Carcinogen-DNA adducts in human breast tissue.

Breast cancer is the second leading cause of cancer death among American women. Known risk factors account for only approximately one-third of the 182,000 new cases diagnosed each year in the United States. There is both concern and debate over the contribution of environmental exposures related to lifestyle, occupation, and ambient pollution, particularly in high risk areas such as Long Island...

Use of betapropiolactone to disinfect fresh tissue without impairing antigenicity: method applicable to human immunodeficiency virus (HIV) positive tissue.

A method for inactivating viruses in tissues is reported that does not impair the antigenicity of the Coxsackie virus or of some common tissue antigens, a common problem with standard tissue fixation methods. Tissues can be placed briefly in Betapropiolactone before cryostat sectioning without any adverse effect on preservation or antigen expression. It is suggested that use of Betapropiolacton...

Lymphatics, Lymph and Lymphoid Tissue

In the introductory chapter of this book it is explained that in the lower vertebrates there are lymphatic vessels but no lymph nodes; the lymphoid tissue in fish and amphibia is associated with myeloid tissue in such organs as the intestines, kidney, and liver. Lymph nodes first appear in certain birds, but it is only in mammals that they are invariably present. However, even in mammals, where...